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fibroblast growth kit serum free  (ATCC)


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    ATCC fibroblast growth kit serum free
    Fibroblast Growth Kit Serum Free, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fibroblast growth kit serum free/product/ATCC
    Average 95 stars, based on 126 article reviews
    fibroblast growth kit serum free - by Bioz Stars, 2026-02
    95/100 stars

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    A . Fibroblast cells were cultured in 96-well microplate format prior to being exposed to radiation. After exposure, the cells were incubated for a fixed period of time prior to undergoing Cell Painting. B . Exposed and control cells were fixed, permeabilized, and stained using a set of six Cell Painting dyes. The cells were then imaged in high-throughput using a confocal microscope. The resulting images were segmented to extract phenoprints (i.e., a phenotypic morphological profile) of healthy and radiation exposed cells. Phenoprints were aggregated at the well-level and unsupervised clustering was performed to determine morphological similarities and differences between experimental conditions. Figure adapted from reference . Created in BioRender.

    Journal: bioRxiv

    Article Title: Image-based Morphological Profiling Reveals Signatures of Radiation Exposure

    doi: 10.1101/2025.11.27.691043

    Figure Lengend Snippet: A . Fibroblast cells were cultured in 96-well microplate format prior to being exposed to radiation. After exposure, the cells were incubated for a fixed period of time prior to undergoing Cell Painting. B . Exposed and control cells were fixed, permeabilized, and stained using a set of six Cell Painting dyes. The cells were then imaged in high-throughput using a confocal microscope. The resulting images were segmented to extract phenoprints (i.e., a phenotypic morphological profile) of healthy and radiation exposed cells. Phenoprints were aggregated at the well-level and unsupervised clustering was performed to determine morphological similarities and differences between experimental conditions. Figure adapted from reference . Created in BioRender.

    Article Snippet: Primary Dermal Fibroblasts (HDFn, PCS-201-010), Fibroblast Basal Medium, Fibroblast Growth Kit, Trypsin-EDTA for Primary Cells, Trypsin Neutralizing Solution, and Dulbecco’s Phosphate Buffered Saline were purchased from ATCC (Manassas, VA).

    Techniques: Cell Culture, Incubation, Control, Staining, High Throughput Screening Assay, Microscopy

    A. Representative Cell Painting images of fibroblasts exposed to, or shielded from, two doses of radiation. Samples were incubated for 4, 24, or 48 hours. Three of the six imaged channels are shown (blue = DNA, green = RNA, yellow = Actin). Images are from Experiment 2 B. Median number of segmented cells per image post-QC. Error bars denote interquartile range (IQR).

    Journal: bioRxiv

    Article Title: Image-based Morphological Profiling Reveals Signatures of Radiation Exposure

    doi: 10.1101/2025.11.27.691043

    Figure Lengend Snippet: A. Representative Cell Painting images of fibroblasts exposed to, or shielded from, two doses of radiation. Samples were incubated for 4, 24, or 48 hours. Three of the six imaged channels are shown (blue = DNA, green = RNA, yellow = Actin). Images are from Experiment 2 B. Median number of segmented cells per image post-QC. Error bars denote interquartile range (IQR).

    Article Snippet: Primary Dermal Fibroblasts (HDFn, PCS-201-010), Fibroblast Basal Medium, Fibroblast Growth Kit, Trypsin-EDTA for Primary Cells, Trypsin Neutralizing Solution, and Dulbecco’s Phosphate Buffered Saline were purchased from ATCC (Manassas, VA).

    Techniques: Incubation